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mm17t  (Sino Biological)


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    Structured Review

    Sino Biological mm17t
    (A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control <t>MM17T.</t> AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.
    Mm17t, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mm17t/SARS-CoV-2+Spike+Neutralizing+Antibody%2C+Mouse+Mab/bio_rxiv__2024__08__13__607736-136-0-1
    Average 91 stars, based on 3 article reviews
    mm17t - by Bioz Stars, 2026-09
    91/100 stars

    Images

    1) Product Images from "AAB-seq: An antigen-specific and affinity-readable high-throughput BCR sequencing method"

    Article Title: AAB-seq: An antigen-specific and affinity-readable high-throughput BCR sequencing method

    Journal: bioRxiv

    doi: 10.1101/2024.08.13.607736

    (A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.
    Figure Legend Snippet: (A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.

    Techniques Used: Sequencing, Expressing, Enzyme-linked Immunosorbent Assay, Neutralization, Activity Assay, Positive Control

    (A) Neutralization assay data of pseudovirus neutralization(%) for SARS-CoV-2 WT. Data are represented as means ± SDs, n=3. (B) Antibodies were tested for antibody-dependent cellular phagocytosis activity against SARS-CoV-2 RBD, compared to positive control MM17T. Phagocytosis score (see Methods) is shown on the y-axis and antibody concentration is shown on the x-axis. Data are represented as means ± SDs, n=3. (C) Confirmation of phagocytosis by Confocal microscopy images. Nuclei are stained blue, beads red, and membrane in green with Concanavalin A(FITC-ConA). (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. Complement deposition score (see Methods) is shown on the y-axis and antibody concentration is shown on the x axis. Data are represented as means ± SDs, n=3. (E) Confocal microscopy images confirm the detection of complement deposition on fluorescent beads. Beads are stained red, and detection antibody in green with FITC-C3b antibody.
    Figure Legend Snippet: (A) Neutralization assay data of pseudovirus neutralization(%) for SARS-CoV-2 WT. Data are represented as means ± SDs, n=3. (B) Antibodies were tested for antibody-dependent cellular phagocytosis activity against SARS-CoV-2 RBD, compared to positive control MM17T. Phagocytosis score (see Methods) is shown on the y-axis and antibody concentration is shown on the x-axis. Data are represented as means ± SDs, n=3. (C) Confirmation of phagocytosis by Confocal microscopy images. Nuclei are stained blue, beads red, and membrane in green with Concanavalin A(FITC-ConA). (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. Complement deposition score (see Methods) is shown on the y-axis and antibody concentration is shown on the x axis. Data are represented as means ± SDs, n=3. (E) Confocal microscopy images confirm the detection of complement deposition on fluorescent beads. Beads are stained red, and detection antibody in green with FITC-C3b antibody.

    Techniques Used: Neutralization, Activity Assay, Positive Control, Concentration Assay, Confocal Microscopy, Staining, Membrane

    Related Articles

    Positive Control:

    Article Title: AAB-seq: An antigen-specific and affinity-readable high-throughput BCR sequencing method
    Article Snippet: .. MM17T (Sino biological, Cat:40592-MM17T) was used as a positive control. .. Antibodies and beads were incubated with THP-1 cells overnight, fixed and interrogated on the Beckman Coulter CytoFLEX SRT.



    Similar Products

    mm17t  (Sino Biological)
    91
    Sino Biological mm17t
    (A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control <t>MM17T.</t> AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.
    Mm17t, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mm17t/SARS-CoV-2+Spike+Neutralizing+Antibody%2C+Mouse+Mab/bio_rxiv__2024__08__13__607736-136-0-1
    Average 91 stars, based on 1 article reviews
    mm17t - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.

    Journal: bioRxiv

    Article Title: AAB-seq: An antigen-specific and affinity-readable high-throughput BCR sequencing method

    doi: 10.1101/2024.08.13.607736

    Figure Lengend Snippet: (A) All B cells (dots) recovered that had paired heavy/light chain sequencing information and antigen/second antibody reactivity information from the AAB-seq experiment for SARS-COV-2 RBD antibodies (n = 3,712) are shown, with CLR(RBD_UMI) (x axis) and AAB-scores (y axis). Antibodies selected dispersedly for expression and validation (colorized dots) are colored by the LG(ELISA_AUC) scores from lowest (purple) to highest (red). (B) Neutralization assays were performed using pseudotyped viruses displaying the S protein from indicated SARS-CoV-2 WT. The values are means ± SD, n = 3. (C) Antibodies were tested for antibody-dependent cellular phagocytosis activity (ADCP) against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the trogocytosis score is shown, calculated from data in . Data are represented as means ± SDs, n=3. (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. AUC of the C3b deposition score is shown, calculated from data in . Data are represented as means ± SDs, n=3.

    Article Snippet: MM17T (Sino biological, Cat:40592-MM17T) was used as a positive control.

    Techniques: Sequencing, Expressing, Enzyme-linked Immunosorbent Assay, Neutralization, Activity Assay, Positive Control

    (A) Neutralization assay data of pseudovirus neutralization(%) for SARS-CoV-2 WT. Data are represented as means ± SDs, n=3. (B) Antibodies were tested for antibody-dependent cellular phagocytosis activity against SARS-CoV-2 RBD, compared to positive control MM17T. Phagocytosis score (see Methods) is shown on the y-axis and antibody concentration is shown on the x-axis. Data are represented as means ± SDs, n=3. (C) Confirmation of phagocytosis by Confocal microscopy images. Nuclei are stained blue, beads red, and membrane in green with Concanavalin A(FITC-ConA). (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. Complement deposition score (see Methods) is shown on the y-axis and antibody concentration is shown on the x axis. Data are represented as means ± SDs, n=3. (E) Confocal microscopy images confirm the detection of complement deposition on fluorescent beads. Beads are stained red, and detection antibody in green with FITC-C3b antibody.

    Journal: bioRxiv

    Article Title: AAB-seq: An antigen-specific and affinity-readable high-throughput BCR sequencing method

    doi: 10.1101/2024.08.13.607736

    Figure Lengend Snippet: (A) Neutralization assay data of pseudovirus neutralization(%) for SARS-CoV-2 WT. Data are represented as means ± SDs, n=3. (B) Antibodies were tested for antibody-dependent cellular phagocytosis activity against SARS-CoV-2 RBD, compared to positive control MM17T. Phagocytosis score (see Methods) is shown on the y-axis and antibody concentration is shown on the x-axis. Data are represented as means ± SDs, n=3. (C) Confirmation of phagocytosis by Confocal microscopy images. Nuclei are stained blue, beads red, and membrane in green with Concanavalin A(FITC-ConA). (D) Antibodies were tested for antibody-dependent complement deposition (ADCD) activity against SARS-CoV-2 RBD, compared to positive control MM17T. Complement deposition score (see Methods) is shown on the y-axis and antibody concentration is shown on the x axis. Data are represented as means ± SDs, n=3. (E) Confocal microscopy images confirm the detection of complement deposition on fluorescent beads. Beads are stained red, and detection antibody in green with FITC-C3b antibody.

    Article Snippet: MM17T (Sino biological, Cat:40592-MM17T) was used as a positive control.

    Techniques: Neutralization, Activity Assay, Positive Control, Concentration Assay, Confocal Microscopy, Staining, Membrane